# User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/09

(Difference between revisions)
 Revision as of 10:16, 9 July 2013 (view source) (→Entry title)← Previous diff Current revision (09:39, 16 July 2013) (view source) (→BSA and HAuCl4 Solutions) (6 intermediate revisions not shown.) Line 8: Line 8: ==BSA and HAuCl4 Solutions== ==BSA and HAuCl4 Solutions== - MW = 393.83 g/mol + *Au solution of HAuCl4:3H2O - 3 mol H20: 1 mol HAuCl4 + **MW = 393.83 g/mol + **in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O + ***actual amount used: 10.01mg + ***therefore, I need 11.0048mL H2O + *89-98,000 BSA solution + **MW = 66776g + **in 20mL 15μM solution, I need 20.032mg BSA + ***actual amount used: 20.120mg BSA + ***therefore, I need 20.0879mL of H2O + ==Gold:BSA ratios== + *Ratio 1: 60 Au:BSA + **fixed: .25mM Au + **new BSA: 0.00417mM + **solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O + *Ratio 2: 90 Au:BSA + **fixed: .25mM Au + **new BSA: 0.00278mM + **solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O + *Ratio 3: 120 Au:BSA + **fixed: .25mM Au + **new BSA: 0.00208mM + **solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O + *Ratio 4: 150 Au:BSA + **fixed: .25mM Au + **new BSA: 0.00167mM + **solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O + *All solutions put into the 80°C oven at 12:07PM and taken out at 4PM

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## BSA and HAuCl4 Solutions

• Au solution of HAuCl4:3H2O
• MW = 393.83 g/mol
• in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
• actual amount used: 10.01mg
• therefore, I need 11.0048mL H2O
• 89-98,000 BSA solution
• MW = 66776g
• in 20mL 15μM solution, I need 20.032mg BSA
• actual amount used: 20.120mg BSA
• therefore, I need 20.0879mL of H2O

## Gold:BSA ratios

• Ratio 1: 60 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00417mM
• solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O
• Ratio 2: 90 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00278mM
• solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O
• Ratio 3: 120 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00208mM
• solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O
• Ratio 4: 150 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00167mM
• solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
• All solutions put into the 80°C oven at 12:07PM and taken out at 4PM