User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 31: Line 31:
==Gel==
==Gel==
*10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
*10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
**This solution was heated and stirred for approximately 50 minutes until a homogeneous gel remained
**This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
**1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H<sub>2</sub>O solution
**After stirring the new solution, it was placed in the oven




Revision as of 09:46, 13 May 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Nanoparticle Fiber

  • Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
    • Concentrations:
      • Au = .25 mM
      • BSA = 1.785 μM
    • Amounts:
      • Au = .4924 mg
      • BSA = 1.19 mg
  • Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
    • Theoretical Amounts:
      • Au = 4.924 mg
      • BSA = 11.9 mg
    • Actual amounts used:
      • Au = 5.3 mg
      • BSA = 12.6 mg
    • Adjusted mL based on extra amounts:
      • Au solution = 10.76mL
      • BSA solution = 10.57mL
  • 1mL was siphoned from each solution and added to 8mL of purified water.
  • The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.

Gel

  • 10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
    • This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
    • 1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H2O solution
    • After stirring the new solution, it was placed in the oven



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Stephanie_Tietz/Notebook/Stephanies_Notebook/2013/05/13&oldid=697825"