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Nanoparticle Fiber
- Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
- Concentrations:
- Au = .25 mM
- BSA = 1.785 μM
- Amounts:
- Au = .4924 mg
- BSA = 1.19 mg
- Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
- Theoretical Amounts:
- Au = 4.924 mg
- BSA = 11.9 mg
- Actual amounts used:
- Au = 5.3 mg
- BSA = 12.6 mg
- Adjusted mL based on extra amounts:
- Au solution = 10.76mL
- BSA solution = 10.57mL
- 1mL was siphoned from each solution and added to 8mL of purified water.
- The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.
Gel
- 10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
- This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
- 1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H2O solution
- After stirring the new solution, it was placed in the oven
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