User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Nanoparticle Fiber)
Line 9: Line 9:
*Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
*Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
**Concentrations:
**Concentrations:
-
Au = .25 mM,
+
***Au = .25 mM
-
BSA = 1.785 μM
+
***BSA = 1.785 μM
-
 
+
**Amounts:
**Amounts:
-
Au = .4924 mg,
+
***Au = .4924 mg
-
BSA = 1.19 mg
+
***BSA = 1.19 mg
-
 
+
*Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
*Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
**Theoretical Amounts:
**Theoretical Amounts:
-
Au = 4.924 mg,
+
***Au = 4.924 mg
-
BSA = 11.9 mg
+
***BSA = 11.9 mg
-
 
+
**Actual amounts used:
**Actual amounts used:
-
Au = 5.3 mg,
+
***Au = 5.3 mg
-
BSA = 12.6 mg
+
***BSA = 12.6 mg
-
 
+
**Adjusted mL based on extra amounts:
**Adjusted mL based on extra amounts:
-
Au solution = 10.76mL,
+
***Au solution = 10.76mL
-
BSA solution = 10.57mL
+
***BSA solution = 10.57mL
*1mL was siphoned from each solution and added to 8mL of purified water.
*1mL was siphoned from each solution and added to 8mL of purified water.

Revision as of 10:22, 13 May 2013

Project name Main project page
Next entry

Nanoparticle Fiber

  • Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
    • Concentrations:
      • Au = .25 mM
      • BSA = 1.785 μM
    • Amounts:
      • Au = .4924 mg
      • BSA = 1.19 mg
  • Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
    • Theoretical Amounts:
      • Au = 4.924 mg
      • BSA = 11.9 mg
    • Actual amounts used:
      • Au = 5.3 mg
      • BSA = 12.6 mg
    • Adjusted mL based on extra amounts:
      • Au solution = 10.76mL
      • BSA solution = 10.57mL
  • 1mL was siphoned from each solution and added to 8mL of purified water.
  • The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.



Personal tools