# User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13

(Difference between revisions)
 Revision as of 11:20, 13 May 2013 (view source) (→Nanoparticle Fiber)← Previous diff Revision as of 11:22, 13 May 2013 (view source)Next diff → Line 9: Line 9: *Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA *Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA **Concentrations: **Concentrations: - Au = .25 mM, + ***Au = .25 mM - BSA = 1.785 μM + ***BSA = 1.785 μM - + **Amounts: **Amounts: - Au = .4924 mg, + ***Au = .4924 mg - BSA = 1.19 mg + ***BSA = 1.19 mg - + *Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions *Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions **Theoretical Amounts: **Theoretical Amounts: - Au = 4.924 mg, + ***Au = 4.924 mg - BSA = 11.9 mg + ***BSA = 11.9 mg - + **Actual amounts used: **Actual amounts used: - Au = 5.3 mg, + ***Au = 5.3 mg - BSA = 12.6 mg + ***BSA = 12.6 mg - + **Adjusted mL based on extra amounts: **Adjusted mL based on extra amounts: - Au solution = 10.76mL, + ***Au solution = 10.76mL - BSA solution = 10.57mL + ***BSA solution = 10.57mL *1mL was siphoned from each solution and added to 8mL of purified water. *1mL was siphoned from each solution and added to 8mL of purified water.

## Revision as of 11:22, 13 May 2013

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## Nanoparticle Fiber

• Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
• Concentrations:
• Au = .25 mM
• BSA = 1.785 μM
• Amounts:
• Au = .4924 mg
• BSA = 1.19 mg
• Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
• Theoretical Amounts:
• Au = 4.924 mg
• BSA = 11.9 mg
• Actual amounts used:
• Au = 5.3 mg
• BSA = 12.6 mg
• Adjusted mL based on extra amounts:
• Au solution = 10.76mL
• BSA solution = 10.57mL
• 1mL was siphoned from each solution and added to 8mL of purified water.
• The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.