# User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13

(Difference between revisions)
 Revision as of 11:18, 13 May 2013 (view source)← Previous diff Revision as of 11:20, 13 May 2013 (view source) (→Nanoparticle Fiber)Next diff → Line 8: Line 8: ==Nanoparticle Fiber== ==Nanoparticle Fiber== *Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA *Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA - Concentrations: + **Concentrations: - Au = .25 mM + Au = .25 mM, BSA = 1.785 μM BSA = 1.785 μM - Amounts: + **Amounts: - Au = .4924 mg + Au = .4924 mg, BSA = 1.19 mg BSA = 1.19 mg *Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions *Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions - Theoretical Amounts: + **Theoretical Amounts: - Au = 4.924 mg + Au = 4.924 mg, BSA = 11.9 mg BSA = 11.9 mg - Actual amounts used: + **Actual amounts used: - Au = 5.3 mg + Au = 5.3 mg, BSA = 12.6 mg BSA = 12.6 mg - Adjusted mL based on extra amounts: + **Adjusted mL based on extra amounts: - Au solution = 10.76mL + Au solution = 10.76mL, BSA solution = 10.57mL BSA solution = 10.57mL

## Revision as of 11:20, 13 May 2013

Project name Main project page
Next entry

## Nanoparticle Fiber

• Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
• Concentrations:

Au = .25 mM, BSA = 1.785 μM

• Amounts:

Au = .4924 mg, BSA = 1.19 mg

• Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
• Theoretical Amounts:

Au = 4.924 mg, BSA = 11.9 mg

• Actual amounts used:

Au = 5.3 mg, BSA = 12.6 mg

• Adjusted mL based on extra amounts:

Au solution = 10.76mL, BSA solution = 10.57mL

• 1mL was siphoned from each solution and added to 8mL of purified water.
• The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.