# User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13

(Difference between revisions)
 Revision as of 11:14, 13 May 2013 (view source)← Previous diff Revision as of 11:18, 13 May 2013 (view source)Next diff → Line 16: Line 16: BSA = 1.19 mg BSA = 1.19 mg - *Amounts needed multiplied by 10 to get 10 times the concentration, to later siphon out 1mL from each diluted solution + *Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions Theoretical Amounts: Theoretical Amounts: Au = 4.924 mg Au = 4.924 mg

## Revision as of 11:18, 13 May 2013

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## Nanoparticle Fiber

• Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA

Concentrations: Au = .25 mM BSA = 1.785 μM

Amounts: Au = .4924 mg BSA = 1.19 mg

• Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions

Theoretical Amounts: Au = 4.924 mg BSA = 11.9 mg

Actual amounts used: Au = 5.3 mg BSA = 12.6 mg

Adjusted mL based on extra amounts: Au solution = 10.76mL BSA solution = 10.57mL

• 1mL was siphoned from each solution and added to 8mL of purified water.
• The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.