User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13: Difference between revisions
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== | ==Nanoparticle Fiber== | ||
* | * Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA | ||
Concentrations: | |||
Au = .25 mM | |||
BSA = 1.785 μM | |||
Amounts: | |||
Au = .4924 mg | |||
BSA = 1.19 mg | |||
*Amounts needed multiplied by 10 to get 10 times the concentration, to later siphon out 1mL from each diluted solution | |||
Theoretical Amounts: | |||
Au = 4.924 mg | |||
BSA = 11.9 mg | |||
Actual amounts used: | |||
Au = 5.3 mg | |||
BSA = 12.6 mg | |||
Adjusted mL based on extra amounts: | |||
Au solution = 10.76mL | |||
BSA solution = 10.57mL | |||
*1mL was siphoned from each solution and added to 8mL of purified water. | |||
*The 10mL Au+BSA+H<sub>2</sub>O solution was then put into the oven for approximately 4 hours. | |||
Revision as of 08:12, 13 May 2013
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Nanoparticle Fiber
Concentrations: Au = .25 mM BSA = 1.785 μM Amounts: Au = .4924 mg BSA = 1.19 mg
Theoretical Amounts: Au = 4.924 mg BSA = 11.9 mg Actual amounts used: Au = 5.3 mg BSA = 12.6 mg Adjusted mL based on extra amounts: Au solution = 10.76mL BSA solution = 10.57mL
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