# User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13

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 Revision as of 10:18, 13 May 2013 (view source)← Previous diff Current revision (11:48, 13 May 2013) (view source) (→Nanoparticle Fiber) (7 intermediate revisions not shown.) Line 8: Line 8: ==Nanoparticle Fiber== ==Nanoparticle Fiber== *Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA *Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA - Concentrations: + **Concentrations: - Au = .25 mM + ***Au = .25 mM - BSA = 1.785 μM + ***BSA = 1.785 μM - + **Amounts: - Amounts: + ***Au = .4924 mg - Au = .4924 mg + ***BSA = 1.19 mg - BSA = 1.19 mg + - + *Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions *Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions - Theoretical Amounts: + **Theoretical Amounts: - Au = 4.924 mg + ***Au = 4.924 mg - BSA = 11.9 mg + ***BSA = 11.9 mg + **Actual amounts used: + ***Au = 5.3 mg + ***BSA = 12.6 mg + **Adjusted mL based on extra amounts: + ***Au solution = 10.76mL + ***BSA solution = 10.57mL - Actual amounts used: + *1mL was siphoned from each stock solution and added to 8mL of purified water. - Au = 5.3 mg + - BSA = 12.6 mg + - Adjusted mL based on extra amounts: + *The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours. - Au solution = 10.76mL + - BSA solution = 10.57mL + - *1mL was siphoned from each solution and added to 8mL of purified water. + ==Gel== - + *10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight) - *The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours. + **This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained + *1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H2O solution + **After stirring the new solution, it was placed in the oven

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## Nanoparticle Fiber

• Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
• Concentrations:
• Au = .25 mM
• BSA = 1.785 μM
• Amounts:
• Au = .4924 mg
• BSA = 1.19 mg
• Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
• Theoretical Amounts:
• Au = 4.924 mg
• BSA = 11.9 mg
• Actual amounts used:
• Au = 5.3 mg
• BSA = 12.6 mg
• Adjusted mL based on extra amounts:
• Au solution = 10.76mL
• BSA solution = 10.57mL
• 1mL was siphoned from each stock solution and added to 8mL of purified water.
• The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.

## Gel

• 10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
• This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
• 1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H2O solution
• After stirring the new solution, it was placed in the oven