User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13

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Current revision (11:48, 13 May 2013) (view source)
(Nanoparticle Fiber)
 
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==Nanoparticle Fiber==
==Nanoparticle Fiber==
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* Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
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*Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
-
Concentrations:
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**Concentrations:
-
Au = .25 mM
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***Au = .25 mM
-
BSA = 1.785 μM
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***BSA = 1.785 μM
 +
**Amounts:
 +
***Au = .4924 mg
 +
***BSA = 1.19 mg
 +
*Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
 +
**Theoretical Amounts:
 +
***Au = 4.924 mg
 +
***BSA = 11.9 mg
 +
**Actual amounts used:
 +
***Au = 5.3 mg
 +
***BSA = 12.6 mg
 +
**Adjusted mL based on extra amounts:
 +
***Au solution = 10.76mL
 +
***BSA solution = 10.57mL
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Amounts:
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*1mL was siphoned from each stock solution and added to 8mL of purified water.
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Au = .4924 mg
+
-
BSA = 1.19 mg
+
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*Amounts needed multiplied by 10 to get 10 times the concentration, to later siphon out 1mL from each diluted solution
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*The 10mL Au+BSA+H<sub>2</sub>O solution was then put into the oven for approximately 4 hours.
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Theoretical Amounts:
+
-
Au = 4.924 mg
+
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BSA = 11.9 mg
+
-
Actual amounts used:
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==Gel==
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Au = 5.3 mg
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*10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
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BSA = 12.6 mg
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**This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
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*1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H<sub>2</sub>O solution
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Adjusted mL based on extra amounts:
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**After stirring the new solution, it was placed in the oven
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Au solution = 10.76mL
+
-
BSA solution = 10.57mL
+
-
 
+
-
*1mL was siphoned from each solution and added to 8mL of purified water.
+
-
 
+
-
*The 10mL Au+BSA+H<sub>2</sub>O solution was then put into the oven for approximately 4 hours.
+

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Nanoparticle Fiber

  • Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
    • Concentrations:
      • Au = .25 mM
      • BSA = 1.785 μM
    • Amounts:
      • Au = .4924 mg
      • BSA = 1.19 mg
  • Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
    • Theoretical Amounts:
      • Au = 4.924 mg
      • BSA = 11.9 mg
    • Actual amounts used:
      • Au = 5.3 mg
      • BSA = 12.6 mg
    • Adjusted mL based on extra amounts:
      • Au solution = 10.76mL
      • BSA solution = 10.57mL
  • 1mL was siphoned from each stock solution and added to 8mL of purified water.
  • The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.

Gel

  • 10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
    • This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
  • 1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H2O solution
    • After stirring the new solution, it was placed in the oven



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