User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Nanoparticle Fiber== | ||
* | *Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA | ||
**Concentrations: | |||
***Au = .25 mM | |||
***BSA = 1.785 μM | |||
**Amounts: | |||
***Au = .4924 mg | |||
***BSA = 1.19 mg | |||
*Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions | |||
**Theoretical Amounts: | |||
***Au = 4.924 mg | |||
***BSA = 11.9 mg | |||
**Actual amounts used: | |||
***Au = 5.3 mg | |||
***BSA = 12.6 mg | |||
**Adjusted mL based on extra amounts: | |||
***Au solution = 10.76mL | |||
***BSA solution = 10.57mL | |||
*1mL was siphoned from each stock solution and added to 8mL of purified water. | |||
*The 10mL Au+BSA+H<sub>2</sub>O solution was then put into the oven for approximately 4 hours. | |||
==Gel== | |||
*10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight) | |||
**This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained | |||
*1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H<sub>2</sub>O solution | |||
**After stirring the new solution, it was placed in the oven | |||
Latest revision as of 22:42, 26 September 2017
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Nanoparticle Fiber
Gel
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