User:Sim Huay Ping/Notebook/CBE/08/150/2009/01/13

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(Entry title)
Current revision (10:04, 4 February 2009) (view source)
(AFM sample preparation)
 
Line 9: Line 9:
To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and label as untreated
To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and label as untreated
 +
To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and 6ul of AMP and label as treated
To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and 6ul of AMP and label as treated
 +
Put both microvial into the shaking incubate and leave for 15 mins
Put both microvial into the shaking incubate and leave for 15 mins
 +
Wash, centrifuge and vortex the microvials 3 times with 0.9% NaCl
Wash, centrifuge and vortex the microvials 3 times with 0.9% NaCl
 +
To the pellet after washing, add in 500ul of 50% ethanol and then vortex and centrifuge
To the pellet after washing, add in 500ul of 50% ethanol and then vortex and centrifuge
 +
To the pellet after washing with 50% ethanol, add in 500ul of 90% ethanol and then vortex and centrifuge
To the pellet after washing with 50% ethanol, add in 500ul of 90% ethanol and then vortex and centrifuge
 +
Pipette 10ul of each treated and the untreated mixture onto the microscope glass. Spread the samples beside each other
Pipette 10ul of each treated and the untreated mixture onto the microscope glass. Spread the samples beside each other
 +
Leave the sample to dry for 15 mins
Leave the sample to dry for 15 mins

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AFM sample preparation

To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and label as untreated

To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and 6ul of AMP and label as treated

Put both microvial into the shaking incubate and leave for 15 mins

Wash, centrifuge and vortex the microvials 3 times with 0.9% NaCl

To the pellet after washing, add in 500ul of 50% ethanol and then vortex and centrifuge

To the pellet after washing with 50% ethanol, add in 500ul of 90% ethanol and then vortex and centrifuge

Pipette 10ul of each treated and the untreated mixture onto the microscope glass. Spread the samples beside each other

Leave the sample to dry for 15 mins



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