User:Sim Huay Ping/Notebook/CBE/08/150/2009/01/13
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< User:Sim Huay Ping | Notebook | CBE/08/150 | 2009 | 01(Difference between revisions)
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Current revision (11:04, 4 February 2009) (view source) (→AFM sample preparation) |
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To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and label as untreated | To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and label as untreated | ||
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To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and 6ul of AMP and label as treated | To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and 6ul of AMP and label as treated | ||
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Put both microvial into the shaking incubate and leave for 15 mins | Put both microvial into the shaking incubate and leave for 15 mins | ||
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Wash, centrifuge and vortex the microvials 3 times with 0.9% NaCl | Wash, centrifuge and vortex the microvials 3 times with 0.9% NaCl | ||
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To the pellet after washing, add in 500ul of 50% ethanol and then vortex and centrifuge | To the pellet after washing, add in 500ul of 50% ethanol and then vortex and centrifuge | ||
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To the pellet after washing with 50% ethanol, add in 500ul of 90% ethanol and then vortex and centrifuge | To the pellet after washing with 50% ethanol, add in 500ul of 90% ethanol and then vortex and centrifuge | ||
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Pipette 10ul of each treated and the untreated mixture onto the microscope glass. Spread the samples beside each other | Pipette 10ul of each treated and the untreated mixture onto the microscope glass. Spread the samples beside each other | ||
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Leave the sample to dry for 15 mins | Leave the sample to dry for 15 mins | ||
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AFM sample preparationTo 100ml of bacteria innoculum in a microvial, add 100ul of AMP and label as untreated To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and 6ul of AMP and label as treated Put both microvial into the shaking incubate and leave for 15 mins Wash, centrifuge and vortex the microvials 3 times with 0.9% NaCl To the pellet after washing, add in 500ul of 50% ethanol and then vortex and centrifuge To the pellet after washing with 50% ethanol, add in 500ul of 90% ethanol and then vortex and centrifuge Pipette 10ul of each treated and the untreated mixture onto the microscope glass. Spread the samples beside each other Leave the sample to dry for 15 mins
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