User:Sim Huay Ping/Notebook/CBE/08/150/2008/12/15: Difference between revisions
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== | ==Preparation of mediums and validating results== | ||
Prepared Nutrient Agar in 400ml bottles | |||
Prepared Mueller Hinton Broth | |||
Prepared HEPES buffer (Weigh 0.74g of solid KCl, 0.36g of solid glucose, 500ul of HEPES | |||
solution in 100ml of DI water) | |||
Prepared Physiological Buffered Saline (PBS) | |||
Prepared Saborand Dextrose Broth | |||
Brought to autoclave | |||
There were no signs of bacteria growth on the kinetics experiment done last week | |||
Changed the PBS used in the kinetics experiment to MHB to minimize death of the bacteria, which could be caused by the waiting time in between the procedures | |||
Revision as of 03:28, 7 January 2009
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Preparation of mediums and validating resultsPrepared Nutrient Agar in 400ml bottles Prepared Mueller Hinton Broth Prepared HEPES buffer (Weigh 0.74g of solid KCl, 0.36g of solid glucose, 500ul of HEPES solution in 100ml of DI water) Prepared Physiological Buffered Saline (PBS) Prepared Saborand Dextrose Broth Brought to autoclave
Changed the PBS used in the kinetics experiment to MHB to minimize death of the bacteria, which could be caused by the waiting time in between the procedures
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