User:Sim Huay Ping/Notebook/CBE/08/150/2008/12/15

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(Autocreate 2008/12/15 Entry for User:Sim_Huay_Ping/Notebook/CBE/08/150)
Current revision (05:28, 7 January 2009) (view source)
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==Entry title==
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==Preparation of mediums and validating results==
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* Insert content here...
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Prepared Nutrient Agar in 400ml bottles
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Prepared Mueller Hinton Broth
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Prepared HEPES buffer (Weigh 0.74g of solid KCl, 0.36g of solid glucose, 500ul of HEPES
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solution in 100ml of DI water)
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Prepared Physiological Buffered Saline (PBS)
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Prepared Saborand Dextrose Broth
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Brought to autoclave
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There were no signs of bacteria growth on the kinetics experiment done last week
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Changed the PBS used in the kinetics experiment to MHB to minimize death of the bacteria, which could be caused by the waiting time in between the procedures
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Preparation of mediums and validating results

Prepared Nutrient Agar in 400ml bottles

Prepared Mueller Hinton Broth

Prepared HEPES buffer (Weigh 0.74g of solid KCl, 0.36g of solid glucose, 500ul of HEPES solution in 100ml of DI water)

Prepared Physiological Buffered Saline (PBS)

Prepared Saborand Dextrose Broth

Brought to autoclave


There were no signs of bacteria growth on the kinetics experiment done last week

Changed the PBS used in the kinetics experiment to MHB to minimize death of the bacteria, which could be caused by the waiting time in between the procedures



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