User:Sim Huay Ping/Notebook/CBE/08/150/2008/12/11: Difference between revisions
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== | ==Kinetics experiment on Pseudomonas, using 6.2ul of Zhou’s AMP== | ||
Extracted 500ul of Pseudomonas inoculum left overnight into 5ml of nutrient broth for regeneration | |||
Left the Pseudomonas inoculums in the incubator for 4 hours | |||
Transferred 1000ul of Pseudomonas inoculums into vials | |||
Centrifuged, washed and vortex 3 times with PBS and resuspended the pellet in 1000ul of PBS | |||
Used the UV spectrometer to measure the inoculum sample to 0.05 | |||
Performed kinetics experiment on Pseudomonas, using 6.2ul of Zhou’s AMP | |||
Extracted single colony from bacteria streak plates and transferred the single colony into 5ml of nutrient broth | |||
Left the bacteria inoculums in the incubator overnight | |||
Revision as of 03:24, 7 January 2009
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Kinetics experiment on Pseudomonas, using 6.2ul of Zhou’s AMPExtracted 500ul of Pseudomonas inoculum left overnight into 5ml of nutrient broth for regeneration Left the Pseudomonas inoculums in the incubator for 4 hours Transferred 1000ul of Pseudomonas inoculums into vials Centrifuged, washed and vortex 3 times with PBS and resuspended the pellet in 1000ul of PBS Used the UV spectrometer to measure the inoculum sample to 0.05 Performed kinetics experiment on Pseudomonas, using 6.2ul of Zhou’s AMP
Left the bacteria inoculums in the incubator overnight
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