User:Shahar Sukenik/Notebook/p53CT expression in minimal media/2010/08/02

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

02/08/10: Starter

1. Get colony from petri dish, put in 2-3 ml in LB + amp (1:1000 amp) 2. Grow O/N on shaker, 220 rpm 37C.


Preps for day 3:

1. 2 500 ml Erlenmeyer to prepare M9 media

2. 5 250 ml Erlenmeyer for growing

3. Stocks to prepare mediums:

  • 1. 5xM9 salts
  • 2. 2xYT + NPS
  • 3. 20% glucose
  • 4. 1M MgSO4
  • 5. 1M CaCl2
  • 6. 200 ml DDW


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Shahar_Sukenik/Notebook/p53CT_expression_in_minimal_media/2010/08/02&oldid=439525"