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| Stephanie Lo <br>
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| Harvard College 2010
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| <Br><Br>
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| c/o Harvard College PRISE<br>
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| Leverett Mail Center<br>
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| 28 DeWolfe Street<br>
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| Cambridge, MA 02138
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| <br><br>
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| I have always had a passion for sciences, particularly chemistry, and hope to pursue a joint concentration in Molecular and Cellular Biology and Economics. Eventually, I plan to apply for an MD/PhD program and specialize in digestive disorders.
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| <br><br>
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| <br>
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| NanoDrop results (6/20) performed by Ellenor and Stephanie:<br>
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| (1.5 uL used out of a 30uL elution with nuclease free water)<br>
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| S: 10.9 ng/uL<br>
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| S2: 15.4 ng/uL<br>
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| B: 59.1 ng/uL<br>
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| B1: 25.1 ng/uL<br>
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| <br><br><br>
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| Brainstorming for the two-component systems (really for my own use for now - not expected to be coherent)<br>
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| [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=10653699&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstractPlus Structural comparison of the PhoB and OmpR DNA binding/transactivation domains and the arrangement of PhoB molecules on the phosphate box]<br> | |
| -NMR used to determine 3DE structure of PhoB DNA-binding/transactivation domain. Very similar to OmpR DNA-binding/transactivation domain, except for conformation of the long turn region of PhoB (interaction site for sigma subunit, rather than interaction with alpha subunit for OmpR)
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| <br><br>
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| [http://jb.asm.org.ezp1.harvard.edu/cgi/content/full/185/1/317?view=long&pmid=12486069 Interdomain linkers of homologous response regulators determine their mechanism of action]<br> | |
| Focuses on OmpR and PhoB and, as the title suggests, supports that phosphorylation of sites (particularly N-terminus of both proteins) improves affinity to bind DNA. Isolated C terminus of OmpR is insufficient to productively interact with RNA polymerase. <br>
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| I've been told by some of the lab members that OmpR is an inner-membrane protein and therefore cannot be used for our assays. It seems that we'll have to find another protein ...<br>
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| [http://openwetware.org/wiki/IGEM:Harvard/2007 Harvard iGEM]
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