User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/09/27: Difference between revisions
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*# 72 °C 30 m | *# 72 °C 30 m | ||
* I prepared this today (Fri) and placed in the Chromato. Vincent will run the WP-PCR Mon. morning. | * I prepared this today (Fri) and placed in the Chromato. Vincent will run the WP-PCR Mon. morning. | ||
* Used up the rest of the PfuUltra I DNAP making 225 μL 90% ΦX174 WP-PCR mix | |||
** 185 μL H<sub>2</sub>O | |||
** 25 μL 10X reaction buffer | |||
** 7.5 μL 3.2 nM ΦX174 template (~0.1 nM final) | |||
** 2.5 μL 25 mM dNTPs mix | |||
** 5 μL PfuUltra I DNA polymerase | |||
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Revision as of 12:04, 28 September 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Hypothesis 2: Gene L is necessary for phage propagation.
Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.
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