User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/09/25: Difference between revisions
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* Cell-free expression 80%: | * Cell-free expression 80%: | ||
** 30 μL extract (12May11) | ** 30 μL extract (12May11) | ||
** | ** 1.17 μL water | ||
** | ** 4.5 μL 200 μM malachite green (10 μM final) | ||
** 0.9 μL 100 mM Mg-glutamate (1 mM final) | ** 0.9 μL 100 mM Mg-glutamate (1 mM final) | ||
** 2 μL 3 M K-glutamate (66.7 mM final) | ** 2 μL 3 M K-glutamate (66.7 mM final) | ||
** 22.5 μL 6 mM amino acids mix (1.5 mM final) | ** 22.5 μL 6 mM amino acids mix (1.5 mM final) | ||
** 6.43 μL 14X 3-PGC buffer (1X final) | |||
** 4.5 μL PEG8000 40% (2% final) | ** 4.5 μL PEG8000 40% (2% final) | ||
* Aliquot 6 × 8 μL into 394-well plate and then add plasmid (pBEST-Pr-MGapt-UTR1-deGFP-T500) range: | * Aliquot 6 × 8 μL into 394-well plate and then add plasmid (pBEST-Pr-MGapt-UTR1-deGFP-T500) range: |
Revision as of 15:08, 25 September 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.
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