User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/09/25: Difference between revisions
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** 10 μM: two stong bands at ~100 bp and >100 bp | ** 10 μM: two stong bands at ~100 bp and >100 bp | ||
* Given these results, here's what I think is happening. At too high primer concentration, the ssDNA primers, being complementary strands of WP-PCR, do not unbind, inhibiting PCR. | * Given these results, here's what I think is happening. At too high primer concentration, the ssDNA primers, being complementary strands of WP-PCR, do not unbind, inhibiting PCR. | ||
* I will do one more test over a primer 4 mix of 0.5, 1, 2, 4, and 8 μM. | * I will do one more test over a primer 4 mix of 0, 0.5, 1, 2, 4, and 8 μM. | ||
* Aliquot 6 × 4.5 μL ΦX174 WP-PCR mix (1X reaction buffer, .25 mM dNTPs mix, PfuUltra I DNAP, ~0.1 nM ΦX174 template) | * Aliquot 6 × 4.5 μL ΦX174 WP-PCR mix (1X reaction buffer, .25 mM dNTPs mix, PfuUltra I DNAP, ~0.1 nM ΦX174 template) | ||
*# 0.5 μL 5 water | *# 0.5 μL 5 water |
Revision as of 13:26, 26 September 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Hypothesis 2: Gene L is necessary for phage propagation.
Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.
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