User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/09/21: Difference between revisions
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== | ===Hypothesis 2: Gene L is necessary for phage propagation.=== | ||
* Unfortunately, gel electrophoresis indicated both primer 4 and primer 4 T3485A samples did not work. | |||
* Here is a list of things that changed from yesterday until today: | |||
** new dNTPs mix: shouldn't be a problem, so will continue to use this mix. | |||
** re-programmed PCR program: I checked the machine. The program is as I have it here, so it should work, since the same (or similar) cycling parameters worked previously. | |||
** different primer mixes: this I'm not so sure of. I will create new mixes at 100 μM and 10 μM and try again. | |||
===Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.=== | |||
* Gel electrophoresis indicated BamXI/XhoI double digested DNA of: | |||
*# pBEST-PA-MGapt-T500 ~ 2500 bp dsDNA - OK | |||
*# pBEST-PB-MGapt-T500 ~ 2500 bp dsDNA - OK | |||
*# pBEST-PD-MGapt-T500 ~ 2500 bp dsDNA - OK | |||
*# pBEST-PF-MGapt-T500 ~ 3000-3500 bp dsDNA - not OK | |||
*# pBEST-PG-MGapt-T500 ~ 2500 bp dsDNA - OK | |||
*# pBEST-PL-MGapt-T500 ~ 2500 bp dsDNA - OK | |||
*# pBEST-PL-PA-MGapt-T500 ~ smear - not OK | |||
*# pBEST-NONE-MGapt-T500 ~ 2500 bp dsDNA - OK |
Revision as of 19:38, 23 September 2012
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Hypothesis 2: Gene L is necessary for phage propagation.
Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.
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