User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/09/21: Difference between revisions
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** Linking number adjustment by gyrase / topisomerase IV | ** Linking number adjustment by gyrase / topisomerase IV | ||
** PCR purification | ** PCR purification | ||
===Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.=== | |||
* Gel electrophoresis indicated BamXI/XhoI double digested DNA of: | |||
*# pBEST-PA-MGapt-T500 ~ 2500 bp dsDNA - OK | |||
*# pBEST-PB-MGapt-T500 ~ 2500 bp dsDNA - OK | |||
*# pBEST-PD-MGapt-T500 ~ 2500 bp dsDNA - OK | |||
*# pBEST-PF-MGapt-T500 ~ 3000-3500 bp dsDNA - not OK | |||
*# pBEST-PG-MGapt-T500 ~ 2500 bp dsDNA - OK | |||
*# pBEST-PL-MGapt-T500 ~ 2500 bp dsDNA - OK | |||
*# pBEST-PL-PA-MGapt-T500 ~ smear - not OK | |||
*# pBEST-NONE-MGapt-T500 ~ 2500 bp dsDNA - OK |
Revision as of 16:06, 21 September 2012
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Hypothesis 2: Gene L is necessary for phage propagation.
Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.
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