User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/09/19: Difference between revisions
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===Hypothesis 2: Gene L is necessary for phage propagation.=== | ===Hypothesis 2: Gene L is necessary for phage propagation.=== | ||
* Gel results from WP-PCR on 09/17/2012 showed amplification! So, either it was the 68 °C extension temperature, a bad tube of primer mix, or both. | * Gel results from WP-PCR on 09/17/2012 showed amplification (at ~> 5 kb dsDNA ladder, which is correct)! So, either it was the 68 °C extension temperature, a bad tube of primer mix, or both. | ||
* Next step is to test parallel PFU ligase reaction to repair nicked DNA. | * Next step is to test parallel PFU ligase reaction to repair nicked DNA. | ||
Revision as of 14:11, 19 September 2012
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Hypothesis 2: Gene L is necessary for phage propagation.
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