User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/07/23: Difference between revisions
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===Characterization A: Expression of PHIX174 promoters fused to MGapt.=== | |||
* [http://openwetware.org/wiki/Corum:DNA_Hybridization Hybridized] sense and antisense ssDNAs to make NheI-MGapt-XhoI linker. | |||
===Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.=== | ===Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.=== | ||
* Continuing with | * Continuing with minipreps and sequencing of the transformants specified on [http://openwetware.org/wiki/User:Sean_P_Corum/Notebook/PHIX174_Cell_Free/2012/07/11 07/11/2012.] | ||
===Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.=== | ===Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.=== |
Revision as of 15:34, 23 July 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Characterization A: Expression of PHIX174 promoters fused to MGapt.
Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.
Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.
Hypothesis 2: Gene L is necessary for phage propagation.
Hypothesis 3: Gene L codes for a ~6 kDA protein.
Hypothesis 4: Gene L complexes with cell membrane.
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