User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/07/05: Difference between revisions
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* While I was away, Fillipo attempted to construct these. Either transformants didn't grow, or the negative control ligation showed colonies. | * While I was away, Fillipo attempted to construct these. Either transformants didn't grow, or the negative control ligation showed colonies. | ||
* Started again by making pBEST-SphI//NcoI-deGFP-T500 linker by [http://openwetware.org/wiki/Corum:Digestion digesting] 67nM stock pBEST-OR2OR1Pr-UTR1-deGFP-T500 with SphI and NcoI (20 μL total volume), following by [http://openwetware.org/wiki/Corum:Gel_Purification gel extraction]. | * Started again by making pBEST-SphI//NcoI-deGFP-T500 linker by [http://openwetware.org/wiki/Corum:Digestion digesting] 67nM stock pBEST-OR2OR1Pr-UTR1-deGFP-T500 with SphI and NcoI (20 μL total volume), following by [http://openwetware.org/wiki/Corum:Gel_Purification gel extraction]. | ||
** Gel showed a line at 2.5 kb as expected. Uncut plasmid DNA sample showed farther migration and higher order species, as expected. | |||
===Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.=== | ===Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.=== |
Revision as of 16:10, 5 July 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.
Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.
Hypothesis 2: Gene L is necessary for phage propagation.
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