User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/06/29: Difference between revisions
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* There were no colonies after transformation. Therefore, I decided to characterize all linkers and backbones for those constructs as well as the UTR1-deGFP constructs via [http://openwetware.org/wiki/Corum:DNA_Quantification quantifluore DNA quantification]. [http://openwetware.org/wiki/Image:Quanitfluore_062812.txt Results] were as follows: | * There were no colonies after transformation. Therefore, I decided to characterize all linkers and backbones for those constructs as well as the UTR1-deGFP constructs via [http://openwetware.org/wiki/Corum:DNA_Quantification quantifluore DNA quantification]. [http://openwetware.org/wiki/Image:Quanitfluore_062812.txt Results] were as follows: | ||
** SphI-null- | ** SphI-null-NcoI linker = 73 nM | ||
** SphI-PA-UTRA-NcoI linker = 46 nM | ** SphI-PA-UTRA-NcoI linker = 46 nM | ||
** SphI-PB-UTRB-NcoI linker = 37 nM | ** SphI-PB-UTRB-NcoI linker = 37 nM |
Revision as of 11:49, 29 June 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.
Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.
Hypothesis 2: Gene L is necessary for phage propagation.
To do
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