User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/06/29: Difference between revisions
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**# SphI-PL-UTR1-NcoI linker | **# SphI-PL-UTR1-NcoI linker | ||
**# SphI-PLPA-UTRA-NheI | **# SphI-PLPA-UTRA-NheI | ||
** [http://openwetware.org/wiki/Corum:T4_Ligation Ligate] 0.5 μL pBEST-SphI//NcoI-UTR1-deGFP-T500 (2nM) with 5 μL linkers | ** [http://openwetware.org/wiki/Corum:T4_Ligation Ligate] 0.5 μL pBEST-SphI//NcoI-UTR1-deGFP-T500 (2nM) with 5 μL linkers to create the following constructs: | ||
**# pBEST-PB-UTR1-deGFP-T500 | **# pBEST-PB-UTR1-deGFP-T500 | ||
**# pBEST-PG-UTR1-deGFP-T500 | **# pBEST-PG-UTR1-deGFP-T500 |
Revision as of 11:28, 29 June 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.
Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.
Hypothesis 2: Gene L is necessary for phage propagation.
To do
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