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| |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PHIX174 Cell Free Expression</span>
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| |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
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| ===Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.===
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| * Waiting for sequencing results.
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| ===Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.===
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| * Isolated pBEST-SphI//NcoI-deGFP-T500 vector backbone digest product via [http://openwetware.org/wiki/Corum:Gel_Purification gel extraction].
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| ** Gel eletrophoresis observation: expected 3096 bp and 106 bp dsDNA bands, observed . The larger band was the one purified from the gel.
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| * I [http://openwetware.org/wiki/Corum:T4_Ligation ligated] the different linkers into the pBEST-SphI//NcoI-deGFP-T500 backbone to form pBEST-PX-UTRX-deGGFP-T500, where X = A, B, D, F, G, L, and LA.
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| ===Hypothesis 2: Gene L is necessary for phage propagation.===
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| * Need to design new primers (regular and mutagenic) for amplifying PHIX174 genome.
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| __NOTOC__
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| ==Notes==
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| {{LnNotebookRecentChanges2}}
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| ==Digital Signature==
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| '''SC 16:45, 25 June 2012 (EDT)''':
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| __NOTOC__
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