User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/06/22: Difference between revisions
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===Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.=== | ===Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.=== | ||
* | * Waiting for sequencing results. | ||
===Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.=== | ===Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.=== | ||
* [http://openwetware.org/wiki/ | * Isolated pBEST-SphI//NcoI-deGFP-T500 vector backbone digest product via [http://openwetware.org/wiki/Corum:Gel_Purification gel extraction]. | ||
* I [http://openwetware.org/wiki/Corum:T4_Ligation ligated] the different linkers into the pBEST-SphI//NcoI-deGFP-T500 backbone to form pBEST-PX-UTRX-deGGFP-T500, where X = A, B, D, F, G, L, and LA. | |||
===Hypothesis 2: Gene L is necessary for phage propagation.=== | ===Hypothesis 2: Gene L is necessary for phage propagation.=== | ||
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==Notes== | ==Notes== | ||
{{LnNotebookRecentChanges2}} | {{LnNotebookRecentChanges2}} | ||
==Digital Signature== | |||
'''SC 16:45, 25 June 2012 (EDT)''': | |||
__NOTOC__ | __NOTOC__ |
Revision as of 13:45, 25 June 2012
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Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.
Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.
Hypothesis 2: Gene L is necessary for phage propagation.
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Notes
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Digital Signature
SC 16:45, 25 June 2012 (EDT):