User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/06/18: Difference between revisions

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* The current log for this research project is linked to here: [http://openwetware.org/images/6/62/PHIX174_Research_Log_Jun-18-2012.txt PHIX174_Research_Log_Jun-18-2012.txt]. It will be updated at the beginning of each week.  
* The current log for this research project is linked to here: [http://openwetware.org/images/6/62/PHIX174_Research_Log_Jun-18-2012.txt PHIX174_Research_Log_Jun-18-2012.txt]. It will be updated at the beginning of each week.  


* The first order to the day is to check whether or not I was able to amplify PHIX174 by whole plasmid PCR via gel electrophoresis by the method described in by [http://nar.oxfordjournals.org/content/26/4/1126.short Chen and Ruffner].
* Referring to the research log file, the first order of the day is Hypothesis 3. Over the weekend, I attempted whole plasmid PCR to amplify PHIX174 by whole plasmid PCR (non-mutagenic primers) by the method described in [http://nar.oxfordjournals.org/content/26/4/1126.short Chen and Ruffner]. No amplification observed. One line at 45b observed, corresponding to self-hybridization of the primers.
 
* Result: No amplification observed. One line at 45b observed, corresponding to self-hybridization of the primers.


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Revision as of 13:48, 18 June 2012

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Initial entry for PHIX174 research project

  • Referring to the research log file, the first order of the day is Hypothesis 3. Over the weekend, I attempted whole plasmid PCR to amplify PHIX174 by whole plasmid PCR (non-mutagenic primers) by the method described in Chen and Ruffner. No amplification observed. One line at 45b observed, corresponding to self-hybridization of the primers.