User:Sean E. Lauber: Difference between revisions

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=====Molecular Techniques=====
=====Molecular Techniques=====
*[[Sean_Lauber:Cytokeratin IHC staining| Cytokeratin IHC staining]]
*[[Sean_Lauber:Cytokeratin IHC staining| Cytokeratin IHC staining]]
*[[Sean_Lauber:Fibronectin IHC staining| Fibronectin IHC staining]]
*[[Sean_Lauber:IHC development| IHC development]]
*[[Sean_Lauber:ELISA Development| ELISA Development]]
*[[Sean_Lauber:ELISA Development| ELISA Development]]
*[[Sean_Lauber:Isotype ELISA against Adenovirus | Isotype ELISA against Adenovirus]]
*[[Sean_Lauber:Isotype ELISA against Adenovirus | Isotype ELISA against Adenovirus]]
*[[Sean_Lauber:Generating Adenovirus Lysate | Generating Adenovirus Lysate]]
*[[Sean_Lauber:Generating Cell/Tissue Lysates | Generating Tissue Lysates]]
*[[Sean_Lauber:Bradford Assay | Bradford Assay]]
*[[Sean_Lauber:SDS PAGE Protein Electrophoresis| SDS PAGE Protein Electrophoresis]]
*[[Sean_Lauber:Western Blotting| Western Blotting]]
*[[Sean_Lauber:Flow Cytometry| Flow Cytometry]]
*[[Sean_Lauber:PCR| PCR]]
*[[Sean_Lauber:PCR| PCR]]
*[[Sean_Lauber:Stable Transfection Using Lipofectamine 2000| Stable Transfection Using Lipofectamine 2000]]
*[[Sean_Lauber:Arginase assay| Arginase assay]]
*[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]]
*[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]]
*[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]]
*[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]]
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*[[Sean_Lauber:Reverse Transcription using Superscript II| Reverse Transcription using Superscript II (for Taqman)]]
*[[Sean_Lauber:Reverse Transcription using Superscript II| Reverse Transcription using Superscript II (for Taqman)]]
*[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]]
*[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]]
*[[Sean_Lauber:mOSM ELISA| mOSM ELISA]]


=====Mammalian Cell Culture=====
=====Mammalian Cell Culture=====
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*[[Sean_Lauber:Cytokine Stimulation of Cells| Cytokine Stimulation of Cells]]
*[[Sean_Lauber:Cytokine Stimulation of Cells| Cytokine Stimulation of Cells]]
*[[Sean_Lauber:Culturing of Primary Macrophages| Culturing of Primary Macrophages]]
*[[Sean_Lauber:Culturing of Primary Macrophages| Culturing of Primary Macrophages]]
*[[Sean_Lauber:Adenovirus infection (in vitro)| Adenovirus infection (in vitro)]]


=====Technical=====
=====Technical=====
*[[Sean_Lauber:Agilent Bioanalyzer| Agilent Bioanalyzer]]
*[[Sean_Lauber:Statistics in Graphpad| Statistics in Graphpad]]
*[[Sean_Lauber:AMIRA - Creating Label Fields by Lung Segmentation| AMIRA - Creating Label Fields by Lung Segmentation]]
*[[Sean_Lauber:AMIRA - Creating Label Fields by Lung Segmentation| AMIRA - Creating Label Fields by Lung Segmentation]]
*[[Sean_Lauber:AMIRA - Generating Histograms| AMIRA - Generating Histograms]]
*[[Sean_Lauber:AMIRA - Generating Histograms| AMIRA - Generating Histograms]]
*[[Sean_Lauber:AMIRA - Density Overlays| AMIRA - Density Overlays]]
*[[Sean_Lauber:AMIRA - Density Overlays| AMIRA - Density Overlays]]
*[[Sean_Lauber:AMIRA - Using Snagit| AMIRA - Using Snagit to take pictures]]
*[[Sean_Lauber:ImageJ - Threshold Analysis| ImageJ - Threshold Analysis]]
*[[Sean_Lauber:ImageJ - Threshold Analysis| ImageJ - Threshold Analysis]]
*[[Sean_Lauber:Using the 7900 HT qPCR Machine| Using the 7900 HT qPCR Machine]]
*[[Sean_Lauber:Using the 7900 HT qPCR Machine| Using the 7900 HT qPCR Machine]]
*[[Sean_Lauber:Using the BDCanto Flow Cytometer| Using the BDCanto Flow Cytometer]]
*[[Sean_Lauber:Heat maps in Excel| Heat maps in Excel]]
*[[Sean_Lauber:Heat maps in Excel| Heat maps in Excel]]


=====Animal Work=====
=====Animal Work=====
*[[Sean_Lauber:Broncho-alveolar Lavage| Broncho-alveolar Lavage]]
*[[Sean_Lauber:Broncho-alveolar Lavage| Performing broncho-alveolar lavage to collect alveolar macrophages and BALF]]
*[[Sean_Lauber:Peritoneal Lavage| Peritoneal Lavage]]
*[[Sean_Lauber:Peritoneal Lavage| Performing peritoneal lavage to collect peritoneal macrophages]]
*[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]]
*[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]]
*[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]]
*[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]]
*[[Sean_Lauber:Using the Kras G12D Mouse Model for Lung Cancer| Using the Kras G12D Mouse Model for Lung Cancer]]
*[[Sean_Lauber:Using the Kras G12D Mouse Model for Lung Cancer| Precipitating adenovirus for Kras G12D mice]]
*[[Sean_Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer| Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer]]
*[[Sean_Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer| Clumping LLC cells for the lung cancer model]]
*[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]]
*[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]]
*[[Sean_Lauber:Differential cell staining|Differential cell staining]]
*[[Sean_Lauber:Differential cell staining|Differential cell staining]]
*[[Sean_Lauber:Preparing LLC or B16 cells for injection|Preparing LLC or B16 cells for injection]]


====Joyce Lab Protocols====   
====Joyce Lab Protocols====   
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*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]]
*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]]
*[[Joyce: Ligation| DNA Ligation]]
*[[Joyce: Ligation| DNA Ligation]]
*[[Sean_Lauber: PCR Mutagenesis| PCR Mutagenesis]]
 
==Template excel files (always download the current file)==
 
[http://openwetware.org/images/a/a2/Template_qPCR.xlsx qPCR Template]
 
[http://openwetware.org/wiki/Image:Quadratic_ELISA_equation_and_ELISA_template.xls ELISA template and quadratic formula (for use when equation is not linear)]
 
[http://openwetware.org/wiki/Image:Virus_calculator.xlsx Virus dose calculator]
 
[http://openwetware.org/wiki/Image:Differential_counting_template.xlsx Cell differential counting template]
 
[http://openwetware.org/wiki/Image:BAL_and_Differential_template_%28Prism%29.pzf BAL and Differential Template (Prism)]
 
[http://openwetware.org/wiki/Image:CT_scanning_template.xlsx CT scanning template with example]
 
[http://openwetware.org/wiki/Image:Stimulating_cells_in_vitro_template.xlsx Stimulating cells in vitro template]
 
[http://openwetware.org/wiki/Image:ELISA_spiking_template.xlsx ELISA spiking template]
 
[http://openwetware.org/wiki/Image:Cell_seeding_calculator.xlsx Cell seeding calculator]
 
[http://openwetware.org/wiki/Image:IV_injection_of_cells_calculator.xlsx IV injection of cells calculator]
 
[http://openwetware.org/wiki/Image:Adenovirus_infection_in_vitro.xlsx Adenovirus infection in vitro calculator]


==Links==
==Links==
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*[http://www.fruitfly.org/seq_tools/promoter.html Promoter Predictor]
*[http://www.fruitfly.org/seq_tools/promoter.html Promoter Predictor]
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar]
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar]
*[https://mozy.com/home Backup your data with Mozy]
*[http://www.abcam.com/index.html?pageconfig=resource&rid=11384 Useful IHC optimization tips for paraffin embedded tissue (Abcam)]
 


====Openwetware related====
====Openwetware related====

Latest revision as of 08:09, 22 July 2013

Contact Info

  • sean.laub@gmail.com

Education

  • M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
  • B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
  • DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004

Protocols

Richards Lab Protocols

Molecular Techniques
Mammalian Cell Culture
Technical
Animal Work

Joyce Lab Protocols

Template excel files (always download the current file)

qPCR Template

ELISA template and quadratic formula (for use when equation is not linear)

Virus dose calculator

Cell differential counting template

BAL and Differential Template (Prism)

CT scanning template with example

Stimulating cells in vitro template

ELISA spiking template

Cell seeding calculator

IV injection of cells calculator

Adenovirus infection in vitro calculator

Links

Protocols and general information

Useful links


Openwetware related

Technical guides

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