User:Saroj Pandey/Notebook/SNP PCR optimization/2014/09/17: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Latest revision as of 00:19, 27 September 2017
Project name | Main project page Next entry |
gDNA extraction from buccal cavityCell collection 1. A taster and a non-taster of the compound phenylthiocarbamide (PTC) was identified by tasting papers dipped in PTC solution. 2. Cell suspension from both the taster and the non-taster was collected by mouthwash with 0.9% NaCl solution. 3. The cell suspension was centrifuged at 5000 rpm for 20 minutes. [In the first place, centrifuge needed to be carried out at 12000 rpm for 5 minutes but as the larger centrifuge had the max speed of only 5000 rpm, centrifuge was done for longer time] 4. Supernatant was discarded and the pellet was mixed with 2 ml 0.9% NaCl solution. 5. The suspension was centrifuged at 12000 rpm for 4 minutes. 6. Supernatant was discarded and the pellet was stored. DNA extraction • DNA extraction was carried out using Bioron Tissue DNA kit DNA concentration • Taster : 58.3 ng/µl [260:280 = 1.77] • Non-taster: 25.7 ng/µl [260:280 = 1.74] |