User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/11/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Fluorescence pH 11==
==Fluorescence pH 11==
==Fluorescence Sample Preparation==
The Fluorescence sample was prepared as follows:
[[Image:Calculation temp anneliesefaustino 113016.PNG]]
BSA Fluorescence
# pH 11
# [Au]=0.25 mM
# [BSA]=3.125 uM
# Vf=3000 uL
Stock Concentrations 161108
# [BSA]=  30.4 uM
# [Au]= 3.37 mM
# [NaOH]= 1 M
Volumes for making the solution:
#  308.38 uL BSA
#  225.55 uL Au
#  2466.06 uL HOH
#  3.00 uL NaOH
==Data Analysis==
[[Image:Fluorph11emissionamf.PNG]]
This chart shows the emission maximum of pH 11, [BSA]=3.125 um, [Au]=0.25 mM.
[[Image:Fluorph11integratedamf.PNG]]
This chart shows the integrated intensity of the emission of pH 11, [BSA]=3.125 um, [Au]=0.25 mM.
[[Image:Fluorph11scansparta.PNG]]
This chart shows the first few scans of emission intensity of pH 11, [BSA]=3.125 um, [Au]=0.25 mM.
[[Image:Fluorph11scanspartb.PNG]]
[[Image:Fluorph11scanspartc.PNG]]




Latest revision as of 02:10, 27 September 2017

Project name Main project page
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Fluorescence pH 11

Fluorescence Sample Preparation

The Fluorescence sample was prepared as follows:

BSA Fluorescence

  1. pH 11
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 161108

  1. [BSA]= 30.4 uM
  2. [Au]= 3.37 mM
  3. [NaOH]= 1 M

Volumes for making the solution:

  1. 308.38 uL BSA
  2. 225.55 uL Au
  3. 2466.06 uL HOH
  4. 3.00 uL NaOH

Data Analysis

This chart shows the emission maximum of pH 11, [BSA]=3.125 um, [Au]=0.25 mM.

This chart shows the integrated intensity of the emission of pH 11, [BSA]=3.125 um, [Au]=0.25 mM.

This chart shows the first few scans of emission intensity of pH 11, [BSA]=3.125 um, [Au]=0.25 mM.





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