User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/28: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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automated set up, scanning every 180 seconds (from previously every 5 minutes) | automated set up, scanning every 180 seconds (from previously every 5 minutes) | ||
==Concentrations and Volumes== | |||
BSA Fluorescence Solution | |||
# pH 6 | |||
# [Au]=0.25 mM | |||
# [BSA]=3.125 uM | |||
# Vf=3000 uL | |||
Stock Concentrations 160920 | |||
# [BSA]= 52.6 uM | |||
# [Au]= 4.27 mM | |||
# [HCl]= 1mM | |||
Volumes for making the solution: | |||
# 3 uL HCl | |||
# 294.6 uL BSA | |||
# 292.7 uL Au | |||
# 2409.5 uL H20 | |||
The concentrations and volumes were not accurately scaled to 3 mL. | |||
In the future, we will use automated calculations with a spreadsheet that we uploaded on Dropbox: | |||
"Stock Solution Calculation Template" | |||
volume ratio reductant : metal source (see above) = [BSA] : [Au] = 294.6/292.7 = 1.006 | |||
Hartings ratio: 178/176 = 1.01 | |||
==Data Analysis== | |||
Performed by [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/29 | Dr Matt Hartings:]] | |||
<u>3 mL Sample</u> | |||
# 0.25 mM AuCl3 | |||
## Stock concentration 4.27 mM | |||
## Volume Added: 176 μL | |||
# 3.125 μM BSA | |||
## Stock Concentration 52.6 μM | |||
## Volume Added: 178 μL | |||
# pH 6 | |||
## Stock Concentration 1 mM HCl | |||
## Volume Added: 3 μL | |||
# Volume water added: 2643 μL | |||
<u>UV-Vis of sample</u> | |||
[[Image:20160929 mrh BSA AuNP UVVis.png]] | |||
<u>Fluorescence scans</u> | |||
# Acquisition information | |||
## Excitation: 290 nm, 10 nm slit width | |||
## Emission: 310-540 nm, 10 nm slit width | |||
## 100 nm/minute | |||
## 61 measurements, 180 second delay between measurements | |||
[[Image:20190929 mrh BSAAuNP IntegratedI.png]] | |||
[[Image:20190929 mrh BSAAuNP EmissionMax.png]] | |||
[[Image:20190929 mrh BSAAuNP scans1.png]] | |||
[[Image:20190929 mrh BSAAuNP scans2.png]] | |||
[[Image:20190929 mrh BSAAuNP scans3.png]] | |||
==Notes== | ==Notes== |
Latest revision as of 02:02, 27 September 2017
Project name | Main project page Previous entry Next entry |
Fluorescence Round 3pH 6 automated set up, scanning every 180 seconds (from previously every 5 minutes) Concentrations and VolumesBSA Fluorescence Solution
Stock Concentrations 160920
Volumes for making the solution:
The concentrations and volumes were not accurately scaled to 3 mL. In the future, we will use automated calculations with a spreadsheet that we uploaded on Dropbox: "Stock Solution Calculation Template" volume ratio reductant : metal source (see above) = [BSA] : [Au] = 294.6/292.7 = 1.006 Hartings ratio: 178/176 = 1.01 Data AnalysisPerformed by Dr Matt Hartings:
UV-Vis of sample Fluorescence scans
NotesFind data on Fluorescence computer : C:\WINLAB\DATA save each file individually as ASKII and .txt
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