User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/21: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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pH 4 - 12 | pH 4 - 12 | ||
Fructose concentration : 0. | Fructose concentration : 0.75 mM, 1.25 mM | ||
BSA stock solutions go 4 hours in oven, up to 80 C. | BSA stock solutions go 4 hours in oven, up to 80 C. | ||
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==Solutions== | ==Solutions== | ||
{|border="1" cellpadding="2" | |||
|+ 0.75 mM Fructose | |||
! scope="col" width="50" | pH | |||
! scope="col" width="100" | μL of 1 mM HCl<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 mM NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 M NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of AuCl<sub>3stock</sub> | |||
! scope="col" width="100" | μL of Fructose<sub>stock</sub> | |||
! scope="col" width="100" | μL of BSA<sub>stock</sub> | |||
! scope="col" width="100" | μL of Water | |||
|- | |||
| 4 || 500 || 0 || 0 || 335 || 253 || 316 || 3596 | |||
|- | |||
| 5 || 50 || 0 || 0 || 335 || 253 || 316 || 4046 | |||
|- | |||
| 6 || 5 || 0 || 0 || 335 || 253 || 316 || 4091 | |||
|- | |||
| 7 || 0 || 0 || 0 || 335 || 253 || 316 || 4096 | |||
|- | |||
| 8 || 0 || 5 || 0 || 335 || 253 || 316 || 4091 | |||
|- | |||
| 9 || 0 || 50 || 0 || 335 || 253 || 316 || 4046 | |||
|- | |||
| 10 || 0 || 500 || 0 || 335 || 253 || 316 || 3596 | |||
|- | |||
| 11 || 0 || 0 || 5 || 335 || 253 || 316 || 4091 | |||
|- | |||
| 12 || 0 || 0 || 50 || 335 || 253 || 316 || 4046 | |||
|} | |||
{|border="1" cellpadding="2" | |||
|+ 1.25 mM Fructose | |||
! scope="col" width="50" | pH | |||
! scope="col" width="100" | μL of 1 mM HCl<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 mM NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 M NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of AuCl<sub>3stock</sub> | |||
! scope="col" width="100" | μL of Fructose<sub>stock</sub> | |||
! scope="col" width="100" | μL of BSA<sub>stock</sub> | |||
! scope="col" width="100" | μL of Water | |||
|- | |||
| 4 || 500 || 0 || 0 || 335 || 422 || 316 || 3427 | |||
|- | |||
| 5 || 50 || 0 || 0 || 335 || 422 || 316 || 3877 | |||
|- | |||
| 6 || 5 || 0 || 0 || 335 || 422 || 316 || 3922 | |||
|- | |||
| 7 || 0 || 0 || 0 || 335 || 422 || 316 || 3927 | |||
|- | |||
| 8 || 0 || 5 || 0 || 335 || 422 || 316 || 3922 | |||
|- | |||
| 9 || 0 || 50 || 0 || 335 || 422 || 316 || 3877 | |||
|- | |||
| 10 || 0 || 500 || 0 || 335 || 422 || 316 || 3427 | |||
|- | |||
| 11 || 0 || 0 || 5 || 335 || 422 || 316 || 3922 | |||
|- | |||
| 12 || 0 || 0 || 50 || 335 || 422 || 316 || 3877 | |||
|} | |||
==Objective== | ==Objective== | ||
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==Data Analysis== | ==Data Analysis== | ||
The chart below shows the absorbance scans across 200-800 nm for pH 4-12 with fructose concentration 0.75 mM. | |||
[[Image:160921 UVVIS 0.75.PNG]] | |||
The chart below shows the absorbance scans across 200-800 nm for pH 4-12 with fructose concentration 1.25 mM. | |||
[[Image:160921 UVVIS 1.25.PNG]] | |||
The chart below shows the A(530) for pH 4-12 with fructose concentration 0.75 mM. | |||
[[Image:160921 A530 0.75.PNG]] | |||
The chart below shows the A(530) for pH 4-12 with fructose concentration 1.25 mM. | |||
[[Image:160921 A530 1.25.PNG]] | |||
==Notes== | ==Notes== | ||
pH 11 and 12 seem transparent for both fructose concentrations. This is contradicting our hypothesis from last week, that higher concentrations of fructose facilitate the formation of Au nano particles at higher pH. See last week's observations [[User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/13|here]]. | |||
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Latest revision as of 02:01, 27 September 2017
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UV Vis round 3pH 4 - 12 Fructose concentration : 0.75 mM, 1.25 mM BSA stock solutions go 4 hours in oven, up to 80 C. menu > hill icon > menu > choose program 1 > 1 loop Solutions
ObjectiveDraw conclusions on the concentration of gold nano particles formed, and how the amount formed depends on 1) pH and 2) Fructose concentration. Concentration of nano particles is directly related to absorbance at the relevant wavelength. Protocol
Data AnalysisThe chart below shows the absorbance scans across 200-800 nm for pH 4-12 with fructose concentration 0.75 mM. The chart below shows the absorbance scans across 200-800 nm for pH 4-12 with fructose concentration 1.25 mM. The chart below shows the A(530) for pH 4-12 with fructose concentration 0.75 mM. The chart below shows the A(530) for pH 4-12 with fructose concentration 1.25 mM. NotespH 11 and 12 seem transparent for both fructose concentrations. This is contradicting our hypothesis from last week, that higher concentrations of fructose facilitate the formation of Au nano particles at higher pH. See last week's observations here. |