User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/14: Difference between revisions

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==Data Analysis==
==Data Analysis==


[[Image:BsaMax abs vs time ph 5.png]]
The increase in absorbance is proportionally and time-connected to the formation of AU nano particles.  


[[Image:Max wavelength vs time ph 5.png]]
[[Image:Ocean Optics Ph 5 part 1 160928.PNG]]


The shift in wavelength absorbance indicates the formation of free AU nano particles (linked to the unfolded Protein)


[[Image:BSAAbs 535.15 vs time.png]]
[[Image:Ocean optics ph 5 part 2 160928.PNG]]
 
 
[[Image:Ocean optics ph 5 part 3 160928.PNG]]




Revision as of 09:39, 28 September 2016

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Ocean Optics Round 2

pH 5

Objective

Draw conclusions on reaction speed of nanoparticle formation by measuring absorbance of light, every 2 minutes over a period of 3 hours.


Sample Solution

  1. Stocks
    1. 4.57 mM Au
    2. 53.3 mM BSA
    3. 0.001 mM HCl
    4. DI Water
  1. Volumes Added to Generate Sample
    1. 175.89 uL BSA
    2. 164.11 uL Au
    3. 30 uL HCl
    4. 2629.99 uL HOH

Protocol

The Protocol can be found at the AU Design Lab

Data Analysis

The increase in absorbance is proportionally and time-connected to the formation of AU nano particles.

The shift in wavelength absorbance indicates the formation of free AU nano particles (linked to the unfolded Protein)




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