User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/08/31
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UV VisObjectiveDerive concentration of AU nano particles from light absorbance measured with spectrometer DescriptionCompare absorption of UV-Vis of solutions containing 0.25 mM Gold with
Composition of solutions:
solutions have been prepared by Dr Hartings Data analysisThe general trend, independent of Fructose presence, shows that absorbance at the relevant wavelength (around 530 nm) increases slightly with increasing pH, which means the concentration of nano particles increased slightly. For high pH values, starting at pH = 11, there seem to be no gold nano particles at all. The solutions are transparent, and show no peak in absorption. The difference between 0 mM Fructose and 0.0625 mM Fructose seems to be that latter has higher absorbance for pH 4 and pH 9. For all other pH values, the Fructose does not seem to affect the Au nano concentration significantly. One thing to be careful with when analyzing the peak values is to take into account potential shifts in the base line. The graphs below show both original results, and baseline-corrected results of absorption. The highest concentration of gold nano particles was for 0.0625 and pH 10. NotesCuvette can be cleaned with DI water, 10 percent HCl (because we are using Gold) and soap. Water has to come last. It should be dried with blowing air. Be cautious with air, can shoot off glass pipette at tip of tube if air comes out too strong. for SILVER use nitric acid for cleaning !
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