Fluorescence
Purpose
Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 7
Procedure
- Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
- Au 3.95mM
- (0.25mM)(3.0mL)=(XmL)(3.95mM)
- XmL= .190mL= 190uL Au
- Myoglobin 133uM
- (12.5uM)(3.0mL)=(XmL)(133uM)
- X=0.282mL= 282uL Myoglobin
- Water
- Volume of water= 3000uL- 190uL-282uL= 2528uL
- Absorbance measurements were taken and saved every 3 minutes for 3 hours
- The instrument was set at 80 degrees Celsius
- The scan range parameters were start at 310nm and ending at 540nm
- The emission and exit slit were set at 10nm
Data
Solution for UV-Vis Preparation
Stock Concentrations:
- Myoglobin 133uM
- Silver 25.8mM
- Fructose 33.0mM
0.125mM Fructose
pH
|
μL of 1 mM HNO3stock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AgNO33stock
|
μL of Fructosestock
|
μL of Myoglobinstock
|
μL of Water
|
4 |
500 |
0 |
0 |
48 |
19 |
470 |
3962
|
5 |
50 |
0 |
0 |
48 |
19 |
470 |
4412
|
6 |
5 |
0 |
0 |
48 |
19 |
470 |
4457
|
7 |
0 |
0 |
0 |
48 |
19 |
470 |
4462
|
8 |
0 |
5 |
0 |
48 |
19 |
470 |
4457
|
9 |
0 |
50 |
0 |
48 |
19 |
470 |
4412
|
10 |
0 |
500 |
0 |
48 |
19 |
470 |
3962
|
11 |
0 |
0 |
5 |
48 |
19 |
470 |
4457
|
12 |
0 |
0 |
50 |
48 |
19 |
470 |
4412
|
0.0625 mM Fructose
pH
|
μL of 1 mM HNO3stock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AgNO33stock
|
μL of Fructosestock
|
μL of Myoglobinstock
|
μL of Water
|
4 |
500 |
0 |
0 |
48 |
39 |
470 |
3943
|
5 |
50 |
0 |
0 |
48 |
39 |
470 |
4393
|
6 |
5 |
0 |
0 |
48 |
39 |
470 |
4438
|
7 |
0 |
0 |
0 |
48 |
39 |
470 |
4443
|
8 |
0 |
5 |
0 |
48 |
39 |
470 |
4438
|
9 |
0 |
50 |
0 |
48 |
39 |
470 |
4393
|
10 |
0 |
500 |
0 |
48 |
39 |
470 |
3943
|
11 |
0 |
0 |
5 |
48 |
39 |
470 |
4438
|
12 |
0 |
0 |
50 |
48 |
39 |
470 |
4393
|
|