User:Sara K. Mattson/Notebook/Chemistry 671/2016/10/18: Difference between revisions
From OpenWetWare
(Autocreate 2016/10/18 Entry for User:Sara_K._Mattson/Notebook/Chemistry_671) |
|||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Fluorescence== | ||
==Purpose== | |||
Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 7 | |||
==Procedure== | |||
#Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total | |||
##Au 3.95mM | |||
###(0.25mM)(3.0mL)=(XmL)(3.95mM) | |||
###XmL= .190mL= 190uL Au | |||
##Myoglobin 133uM | |||
###(12.5uM)(3.0mL)=(XmL)(133uM) | |||
###X=0.282mL= 282uL Myoglobin | |||
##Water | |||
###Volume of water= 3000uL- 190uL-282uL= 2528uL | |||
#Absorbance measurements were taken and saved every 3 minutes for 3 hours | |||
#The instrument was set at 80 degrees Celsius | |||
#The scan range parameters were start at 310nm and ending at 540nm | |||
#The emission and exit slit were set at 10nm | |||
Revision as of 10:51, 18 October 2016
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
FluorescencePurposeUse fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 7 Procedure
| |
