User:Sara K. Mattson/Notebook/Chemistry 671/2016/10/18: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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#The scan range parameters were start at 310nm and ending at 540nm | #The scan range parameters were start at 310nm and ending at 540nm | ||
#The emission and exit slit were set at 10nm | #The emission and exit slit were set at 10nm | ||
==Data== | |||
Scans AuNP Myoglobin pH7 | |||
[[Image:Scans of myoglobin AuNP pH7.png|600px]] | |||
Wavelength of Myoglobin AuNp's at pH7 | |||
[[Image:Wavelength myoglobin AuNP pH7.png|600px]] | |||
Maximum Intensity of Myoglobin AuNp's at pH7 | |||
[[Image:Max intensity Myo AuNP pH7.png|600px]] | |||
Integrated Intensity of Myoglobins AuNp's at pH7 | |||
[[Image:Integrated intensity AuNP myo pH7.png|600px]] | |||
==Solution for UV-Vis Preparation== | |||
Stock Concentrations: | |||
#Myoglobin 133uM | |||
#Silver 25.8mM | |||
#Fructose 33.0mM | |||
{|border="1" cellpadding="2" | |||
|+ 0.125mM Fructose | |||
! scope="col" width="50" | pH | |||
! scope="col" width="100" | μL of 1 mM HNO3<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 mM NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 M NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of AgNO3<sub>3stock</sub> | |||
! scope="col" width="100" | μL of Fructose<sub>stock</sub> | |||
! scope="col" width="100" | μL of Myoglobin<sub>stock</sub> | |||
! scope="col" width="100" | μL of Water | |||
|- | |||
| 4 || 500 || 0 || 0 || 48 || 19 || 470 || 3962 | |||
|- | |||
| 5 || 50 || 0 || 0 || 48 || 19 || 470 || 4412 | |||
|- | |||
| 6 || 5 || 0 || 0 || 48 || 19 || 470 || 4457 | |||
|- | |||
| 7 || 0 || 0 || 0 || 48 || 19 || 470 || 4462 | |||
|- | |||
| 8 || 0 || 5 || 0 || 48 || 19 || 470 || 4457 | |||
|- | |||
| 9 || 0 || 50 || 0 || 48 || 19 || 470 || 4412 | |||
|- | |||
| 10 || 0 || 500 || 0 || 48 || 19 || 470 || 3962 | |||
|- | |||
| 11 || 0 || 0 || 5 || 48 || 19 || 470 || 4457 | |||
|- | |||
| 12 || 0 || 0 || 50 || 48 || 19 || 470 || 4412 | |||
|} | |||
{|border="1" cellpadding="2" | |||
|+ 0.25 mM Fructose | |||
! scope="col" width="50" | pH | |||
! scope="col" width="100" | μL of 1 mM HNO3<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 mM NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 M NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of AgNO3<sub>3stock</sub> | |||
! scope="col" width="100" | μL of Fructose<sub>stock</sub> | |||
! scope="col" width="100" | μL of Myoglobin<sub>stock</sub> | |||
! scope="col" width="100" | μL of Water | |||
|- | |||
| 4 || 500 || 0 || 0 || 48 || 39 || 470 || 3943 | |||
|- | |||
| 5 || 50 || 0 || 0 || 48 || 39 || 470 || 4393 | |||
|- | |||
| 6 || 5 || 0 || 0 || 48 || 39 || 470 || 4438 | |||
|- | |||
| 7 || 0 || 0 || 0 || 48 || 39 || 470 || 4443 | |||
|- | |||
| 8 || 0 || 5 || 0 || 48 || 39 || 470 || 4438 | |||
|- | |||
| 9 || 0 || 50 || 0 || 48 || 39 || 470 || 4393 | |||
|- | |||
| 10 || 0 || 500 || 0 || 48 || 39 || 470 || 3943 | |||
|- | |||
| 11 || 0 || 0 || 5 || 48 || 39 || 470 || 4438 | |||
|- | |||
| 12 || 0 || 0 || 50 || 48 || 39 || 470 || 4393 | |||
|} | |||
Latest revision as of 02:05, 27 September 2017
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FluorescencePurposeUse fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 7 Procedure
DataScans AuNP Myoglobin pH7 Wavelength of Myoglobin AuNp's at pH7 Maximum Intensity of Myoglobin AuNp's at pH7 Integrated Intensity of Myoglobins AuNp's at pH7 Solution for UV-Vis PreparationStock Concentrations:
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