User:Sara K. Mattson/Notebook/Chemistry 671/2016/09/20: Difference between revisions
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==Procedure== | ==Procedure== | ||
Solutions were made [[User:Sara K. Mattson/Notebook/Chemistry 671/2016/09/14| 09/14/2016]] | Solutions were made [[User:Sara K. Mattson/Notebook/Chemistry 671/2016/09/14| 09/14/2016]] | ||
The solutions had been in 80C oven for four hours and then sat at room temperature. | |||
UV-Vis spectra were taken of each sample from 200-800nm. | |||
0.0625mM fructose 4-12pH (left to right) | 0.0625mM fructose 4-12pH (left to right) | ||
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[[Image:Myo0.jpg|500px]] | [[Image:Myo0.jpg|500px]] | ||
==Results== | |||
Absorbance v. Wavelength at 0mM Fructose | |||
[[Image:Myoglobin fructose 0mM graph.png|600px]] | |||
Peak at 538nm for 0mM fructose | |||
[[Image:0mm fructose uv vis 538nm.png|600px]] | |||
Absorbance v. Wavelength at 0.0625mM Fructose | |||
[[Image:.0625fructose myoglobin.png|600px]] | |||
Peak at 538nm for 0.0625mM Fructose | |||
[[Image:.0625fructose peak absorbance.png|600px]] | |||
Absorbance v. Wavelength at 0.125mM Fructose | |||
[[Image:0.125mM fructose uvvis.png|600px]] | |||
Peak at 537nm for 0.125mM Fructose | |||
[[Image:Absorbance 537 0.125mM fructose.png|600px]] | |||
==Notes== | |||
The 0mM fructose at pH11, and the .0625mM fructose pH11 had nanoparticles collected at the bottom the test tube and were mixed in before running UV-VIS on the samples. None of the other samples had this. | |||
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Revision as of 11:15, 21 September 2016
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Uv-VisPurposeTo observe the change in absorbance at pH 4-12 for fructose at concentrations 0mM, 0.0625mM and 0.125mM ProcedureSolutions were made 09/14/2016 The solutions had been in 80C oven for four hours and then sat at room temperature. UV-Vis spectra were taken of each sample from 200-800nm. 0.0625mM fructose 4-12pH (left to right)
0.125mM fructose 4-12pH (left to right)
0mM fructose 12-4pH (left to right)
ResultsAbsorbance v. Wavelength at 0mM Fructose
Peak at 538nm for 0mM fructose
Absorbance v. Wavelength at 0.0625mM Fructose
Peak at 538nm for 0.0625mM Fructose
Absorbance v. Wavelength at 0.125mM Fructose
Peak at 537nm for 0.125mM Fructose
NotesThe 0mM fructose at pH11, and the .0625mM fructose pH11 had nanoparticles collected at the bottom the test tube and were mixed in before running UV-VIS on the samples. None of the other samples had this. | |
