User:Samiye Yaman/Notebook/Lab 581/2013/04/03

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==Objections==
==Description==
==Description==
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*The course is designed by American University to experiment different aspects of PVOH films.
 
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*During the course PVOH films with different molecular weight will be produced. Effects of cross-linking, addition of different molecules such as gluteraldehyde, different acids, porphrin will be also tested.
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'''Part A: pH Analysis'''
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*Add 50μL of phenolphthalein to 5mg hydrogel in 1.5mL buffer prepared on 03/29/2013.
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*Add 25μL bromocresol purple to 5mg hydrogels in 1.5mL phosphate buffer.
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* Add 75μL 0.02% cresol red to 5mg hydrogels in 1.5mL phosphate buffer. Allow some time for hydrogels to absorb indicators.
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'''Part B: Create concentration gradient for fluroescence calibration of microscope'''
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* Add 10μL FluoSpheres carboxylate modified microspheres in to 100μL distilled water. This is solution one. Take 10μL from solution 1 and place in to a seperate falcon tube. Add 100μL distilled water. This is solution 2. Solution 3 is created by mixing 10μL of solution from solution 1 and 100μL distilled water. Repeat the same process for creating solution 4 and 5. (Diluting each solution by 10)
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'''Part C: Preparing dilutions of dye containing hydrogels'''
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* Dilute 2mg hydrogels containing 30μL R6G (supernatant was previously removed) with 400μL phosphate buffer (solution 1).  
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-Dilute each solution by half. (take 200μL solution from solution 1 and mix it with 200 phosphate buffer to create solution 3.)
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* Dilute 200μL 2mg of hydrogel solution containing dye in 1.5mL phosphate buffer (from 3/22/13) (marked with heart) with 200μL phosphate buffer (solution 1)
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- Dilute each solution by half. (take 200μL solution from solution 1 and mix it with 200 phosphate buffer to create solution 3.)
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'''Part D: Synthesizing more hydrogels'''
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* Add 0.5g (89,000-98,000 g/mol) PVOH in 6mL phosphate buffer into 2 beakers labelled as A and B.
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* Add 0.5g (89,000-98,000 g/mol) PVOH in 6mL phosphate buffer with 300μL 1mM R6G into 2 beaker labelled as C and D.
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* Place the beakers on a heat block at 110°C for 15 minutes.
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*Let them cool to room temperature.
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*By using blender, mix each hydrogels with 40mL safflower oil for 1-2min on lower setting of blender.
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*Shock freeze the blended hydrogels with oil by using liquid nitrogen and place in freezer at -20°C for 24 hours.
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*After in freezer for 24 hours, take them into room temperature for 4 hours.(cycle 1). The cycles are required.
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==Data==
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*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.
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==Notes==

Current revision

Experimental Chemistry Main project page
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Contents

Objections

Description

Part A: pH Analysis

  • Add 50μL of phenolphthalein to 5mg hydrogel in 1.5mL buffer prepared on 03/29/2013.
  • Add 25μL bromocresol purple to 5mg hydrogels in 1.5mL phosphate buffer.
  • Add 75μL 0.02% cresol red to 5mg hydrogels in 1.5mL phosphate buffer. Allow some time for hydrogels to absorb indicators.

Part B: Create concentration gradient for fluroescence calibration of microscope

  • Add 10μL FluoSpheres carboxylate modified microspheres in to 100μL distilled water. This is solution one. Take 10μL from solution 1 and place in to a seperate falcon tube. Add 100μL distilled water. This is solution 2. Solution 3 is created by mixing 10μL of solution from solution 1 and 100μL distilled water. Repeat the same process for creating solution 4 and 5. (Diluting each solution by 10)

Part C: Preparing dilutions of dye containing hydrogels

  • Dilute 2mg hydrogels containing 30μL R6G (supernatant was previously removed) with 400μL phosphate buffer (solution 1).

-Dilute each solution by half. (take 200μL solution from solution 1 and mix it with 200 phosphate buffer to create solution 3.)

  • Dilute 200μL 2mg of hydrogel solution containing dye in 1.5mL phosphate buffer (from 3/22/13) (marked with heart) with 200μL phosphate buffer (solution 1)

- Dilute each solution by half. (take 200μL solution from solution 1 and mix it with 200 phosphate buffer to create solution 3.)

Part D: Synthesizing more hydrogels

  • Add 0.5g (89,000-98,000 g/mol) PVOH in 6mL phosphate buffer into 2 beakers labelled as A and B.
  • Add 0.5g (89,000-98,000 g/mol) PVOH in 6mL phosphate buffer with 300μL 1mM R6G into 2 beaker labelled as C and D.
  • Place the beakers on a heat block at 110°C for 15 minutes.
  • Let them cool to room temperature.
  • By using blender, mix each hydrogels with 40mL safflower oil for 1-2min on lower setting of blender.
  • Shock freeze the blended hydrogels with oil by using liquid nitrogen and place in freezer at -20°C for 24 hours.
  • After in freezer for 24 hours, take them into room temperature for 4 hours.(cycle 1). The cycles are required.

Data

Notes

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