User:Samiye Yaman/Notebook/Lab 581/2013/03/22

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Part A:Preparing hydrogels for pH analysis

  • Add 1.5ml of water in to a epitest tube with 2mg hydrogel.
  • Centrifuge sample containing hydrogels and crystal violet indicator 3 times (5 minutes, 13200 rpm)

-Measure the absorbance of each supernatant to find the amount of CV that the hydrogels have absorbed

2) Prepare 2 more hydrogel solutions with the following indicators (these will be used to measure dye leakage as well as to monitor pH changes)

a) methyl red (55μL) in 1.5 mL ethanol w/ 2mg hydrogels b) bromophenol blue (30μL) in 1.5 mL water w/ 2mg hydrogels



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