User:Samiye Yaman/Notebook/Lab 581/2013/03/22: Difference between revisions
From OpenWetWare
Samiye Yaman (talk | contribs) |
Samiye Yaman (talk | contribs) |
||
| Line 15: | Line 15: | ||
==Description== | ==Description== | ||
'''Part A''' | '''Part A:Preparing hydrogels for pH analysis''' | ||
*Add 1.5ml of water in to a epitest tube with 2mg hydrogel. | |||
*Centrifuge sample containing hydrogels and crystal violet indicator 3 times (5 minutes, 13200 rpm) | |||
-Measure the absorbance of each supernatant to find the amount of CV that the hydrogels have absorbed | |||
2) Prepare 2 more hydrogel solutions with the following indicators (these will be used to measure dye leakage as well as to monitor pH changes) | |||
a) methyl red (55μL) in 1.5 mL ethanol w/ 2mg hydrogels | |||
b) bromophenol blue (30μL) in 1.5 mL water w/ 2mg hydrogels | |||
==Data== | ==Data== | ||
==Notes== | ==Notes== | ||
Revision as of 08:48, 27 March 2013
 Experimental Chemistry
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
|
ObjectiveDescriptionPart A:Preparing hydrogels for pH analysis
-Measure the absorbance of each supernatant to find the amount of CV that the hydrogels have absorbed 2) Prepare 2 more hydrogel solutions with the following indicators (these will be used to measure dye leakage as well as to monitor pH changes) a) methyl red (55μL) in 1.5 mL ethanol w/ 2mg hydrogels b) bromophenol blue (30μL) in 1.5 mL water w/ 2mg hydrogels DataNotes |
|
