User:Samiye Yaman/Notebook/Lab 581/2013/03/22

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(Autocreate 2013/03/22 Entry for User:Samiye_Yaman/Notebook/Lab_581)
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==Objective==
==Description==
==Description==
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*The course is designed by American University to experiment different aspects of PVOH films.
 
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*During the course PVOH films with different molecular weight will be produced. Effects of cross-linking, addition of different molecules such as gluteraldehyde, different acids, porphrin will be also tested.
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'''Preparing hydrogels for pH analysis'''
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*Add 1.5ml of water in to a epitest tube with 2mg hydrogel.
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*Centrifuge sample containing hydrogels and crystal violet indicator 3 times for 5 minutes at 13200 rpm.
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*By using UV-Vis, measure the absorbance of each supernatant to find the amount of crytal violet in the supernatent. Use the data to find out the amount of crystal violet absorbed by the hydrogels.(Initial amount of indicator added to the epi test tube is 20uL)
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*Prepare 2 more hydrogel solutions (with 1.5 water and 2mg hydrogel) with 55 μL methyl red and 30μL bromophenol blue.
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'''Info on indicators'''
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methyl violet 0-2
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methyl red- 4.4-6.2
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bromocresol purple- 5.2-6.8
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bromophenol blue 6.0-7.6
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cresol red 7.2-8.8
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phenolphthalein- 8.3-10.0
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==Data==
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[[Image:Crystal_violet_leakage.png]]
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*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.
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==Notes==

Current revision

Experimental Chemistry Main project page
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Contents

Objective

Description

Preparing hydrogels for pH analysis

  • Add 1.5ml of water in to a epitest tube with 2mg hydrogel.
  • Centrifuge sample containing hydrogels and crystal violet indicator 3 times for 5 minutes at 13200 rpm.
  • By using UV-Vis, measure the absorbance of each supernatant to find the amount of crytal violet in the supernatent. Use the data to find out the amount of crystal violet absorbed by the hydrogels.(Initial amount of indicator added to the epi test tube is 20uL)
  • Prepare 2 more hydrogel solutions (with 1.5 water and 2mg hydrogel) with 55 μL methyl red and 30μL bromophenol blue.

Info on indicators

methyl violet 0-2

methyl red- 4.4-6.2

bromocresol purple- 5.2-6.8

bromophenol blue 6.0-7.6

cresol red 7.2-8.8

phenolphthalein- 8.3-10.0

Data

Image:Crystal_violet_leakage.png

Notes

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