User:Samiye Yaman/Notebook/Lab 581/2013/03/20: Difference between revisions
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*47ml of more distilled water was added to have 300ml of 25mM tris-HCl buffer. | *47ml of more distilled water was added to have 300ml of 25mM tris-HCl buffer. | ||
'''Part B: Preparing hydrogels for liposome coating''' | '''Part B: Preparing hydrogels for liposome coating''' | ||
* | * 100mg of transfection reagent 1 was dissolved in 5ml choloroform. | ||
*1 ml of the choloroform with transfection reagent 1 was placed under the hood and let it evaporate. | |||
*After the evaporation of the choloroform, 1 ml of phosphate buffer was added to the vial. | |||
*3 freeze-thaw vortex cycles were applied to the vial in order to create multi lamellar vesicles. (30sec freeze with liquid nitrogen, 30 sec thaw in water bath at room temperature, and vortex for 30sec) | |||
*By using Nuclepore Track-Etched Membrane, multi lamellar were converted to uni lamellar vesicles. | |||
*Add 4mg lipid with 2 mg hydrogels.(1. 0.5g PVOH/3mL buffer/6μL R6G/50mL oil 2. 0.5g PVOH/4mL buffer/35mL oil) | |||
*Gently stir for 2 hours. | *Gently stir for 2 hours. |
Revision as of 08:46, 27 March 2013
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectivesPart A: Prepare tris-HCl Buffer
= 0.90855g tris
DescriptionDataNotes |