User:Samiye Yaman/Notebook/Lab 581/2013/03/20: Difference between revisions

From OpenWetWare
< User:Samiye Yaman‎ | Notebook‎ | Lab 581‎ | 2013‎ | 03
Jump to navigationJump to search
(Autocreate 2013/03/20 Entry for User:Samiye_Yaman/Notebook/Lab_581)
 
(fix raw html notebook nav)
 
(3 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 10: Line 10:




==Objectives==
'''Part A: Prepare tris-HCl Buffer'''
*25mmol/L x 1mol/1000mmmol x 1L/1000mL x 300mL x 121.14g/mol
= 0.90855g tris
*Add 250ml of distilled water to the beaker with 0.9094g of tris.
*Add HCl drop by drop until the pH was at 8.07.
*47ml of more distilled water was added to have 300ml of 25mM tris-HCl buffer.
'''Part B: Preparing hydrogels for liposome coating'''
* 100mg of transfection reagent 1 was dissolved in 5ml choloroform.
*1 ml of the choloroform with transfection reagent 1 was placed under the hood and let it evaporate. 
*After the evaporation of the choloroform, 1 ml of phosphate buffer was added to the vial.
*3 freeze-thaw vortex cycles were applied to the vial in order to create multi lamellar vesicles. (30sec freeze with liquid nitrogen, 30 sec thaw in water bath at room temperature, and vortex for 30sec)
*By using Nuclepore Track-Etched Membrane, multi lamellar were converted to uni lamellar vesicles.
*Add 4mg lipid with 2 mg hydrogels.(1. 0.5g PVOH/3mL buffer/6μL R6G/50mL oil 2. 0.5g PVOH/4mL buffer/35mL oil)
*Gently stir for 2 hours.


==Description==
==Description==
   
   
*The course is designed by American University to experiment different aspects of PVOH films.
==Data==
 
*During the course PVOH films with different molecular weight will be produced. Effects of cross-linking, addition of different molecules such as gluteraldehyde, different acids, porphrin will be also tested.


*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.
==Notes==

Latest revision as of 22:34, 26 September 2017

Experimental Chemistry Main project page
Previous entry      Next entry



Objectives

Part A: Prepare tris-HCl Buffer

  • 25mmol/L x 1mol/1000mmmol x 1L/1000mL x 300mL x 121.14g/mol

= 0.90855g tris

  • Add 250ml of distilled water to the beaker with 0.9094g of tris.
  • Add HCl drop by drop until the pH was at 8.07.
  • 47ml of more distilled water was added to have 300ml of 25mM tris-HCl buffer.


Part B: Preparing hydrogels for liposome coating

  • 100mg of transfection reagent 1 was dissolved in 5ml choloroform.
  • 1 ml of the choloroform with transfection reagent 1 was placed under the hood and let it evaporate.
  • After the evaporation of the choloroform, 1 ml of phosphate buffer was added to the vial.
  • 3 freeze-thaw vortex cycles were applied to the vial in order to create multi lamellar vesicles. (30sec freeze with liquid nitrogen, 30 sec thaw in water bath at room temperature, and vortex for 30sec)
  • By using Nuclepore Track-Etched Membrane, multi lamellar were converted to uni lamellar vesicles.
  • Add 4mg lipid with 2 mg hydrogels.(1. 0.5g PVOH/3mL buffer/6μL R6G/50mL oil 2. 0.5g PVOH/4mL buffer/35mL oil)
  • Gently stir for 2 hours.

Description

Data

Notes
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Samiye_Yaman/Notebook/Lab_581/2013/03/20&oldid=1012257"