User:Samiye Yaman/Notebook/Lab 581/2013/03/08: Difference between revisions
Samiye Yaman (talk | contribs) |
Samiye Yaman (talk | contribs) |
||
Line 19: | Line 19: | ||
*2 mg of PVOH with R6G (without lecithin) was placed in 1.5ml of 5 different solution. These solutions were water, phosphate buffer, 1M of NaOH, 1M of HCl and fixant solution. Each PVOH stayed in solution for an hour before centrifuged. 5 minutes centrifuging is followed by UV-Vis measurements. | *2 mg of PVOH with R6G (without lecithin) was placed in 1.5ml of 5 different solution. These solutions were water, phosphate buffer, 1M of NaOH, 1M of HCl and fixant solution. Each PVOH stayed in solution for an hour before centrifuged. 5 minutes centrifuging is followed by UV-Vis measurements of the solutions. | ||
==Data== | ==Data== |
Revision as of 12:22, 22 March 2013
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectivesDescription
Datashows the UV-Vis spectra of the 0.1 g of hydrogel in 5 ml of phosphate buffer (series 1) and 0.1 g of hydrogel with lecithin in 5ml of buffer (series 2). Absorbance of PVOH with lecithin was lower than the observance of PVOH without lecithin. It was indicated that use of lecithin decreases the absorbance of the PVOH. shows the UV-Vis spectra of nanohydrogels in different solutions. Series 1 corresponds to the results obtained from hydrogel being in water, series 2 in phosphate buffer, series 3 in 1 M NaOH, series 4 in 1M of HCl and series 5 in fixant solution. During the synthesis of each hydrogel R6G dye was added. R6G dye should show a peak at 480nm in UV-Vis spectra. Each hydrogel with R6G dye attached stayed in the solution for 2 hours. The results indicate that there was no R6G dye leakage from the hydrogels to each solution as no peaks were observed around 480nm. Notes |