User:Samiye Yaman/Notebook/Lab 581/2013/03/01: Difference between revisions

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==Objections==


==Description==
==Description==
*The course is designed by American University to experiment different aspects of PVOH films.


*During the course PVOH films with different molecular weight will be produced. Effects of cross-linking, addition of different molecules such as gluteraldehyde, different acids, porphrin will be also tested.
'''Part 1'''
*Place a small piece of the hydrogels (synthesized on 22/02/2012) in a water solution.
 
*Observe under the microscope.
 
*Sonicate each samples.
 
*Fluorescence of samples with dyes already attached were measured by using fluorometer.
 
'''Part 2'''
*0.3 g of sample with and without lecithin placed in to 2 different vials.
 
*Add 6ml of water and sonicate the samples.
 
*Take 1 ml of each sample add in to 1.5ml microcentrifuge tubes. Centrifuge samples.( Additionally pipette it up and down to break the larger pieces.)
 
*Add 1μl of R6G to each microcentrifuge tubes to be absorbed by the PVOH.
 
*Fluorescence of samples with dyes already attached were measured by using fluorometer.
 
==Data==
[[Image:0.5gpva_0.25gl_lecithin_in_safflower_oil.png]]


*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.
==Notes==

Revision as of 21:19, 19 March 2013

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Objections

Description

Part 1

  • Place a small piece of the hydrogels (synthesized on 22/02/2012) in a water solution.
  • Observe under the microscope.
  • Sonicate each samples.
  • Fluorescence of samples with dyes already attached were measured by using fluorometer.

Part 2

  • 0.3 g of sample with and without lecithin placed in to 2 different vials.
  • Add 6ml of water and sonicate the samples.
  • Take 1 ml of each sample add in to 1.5ml microcentrifuge tubes. Centrifuge samples.( Additionally pipette it up and down to break the larger pieces.)
  • Add 1μl of R6G to each microcentrifuge tubes to be absorbed by the PVOH.
  • Fluorescence of samples with dyes already attached were measured by using fluorometer.

Data

Notes
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