User:Samiye Yaman/Notebook/Lab 581/2013/02/22

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Experimental Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objections

Description

Part 1

  • Prepare 3 sets of hydrogels with phosphate buffers

A)0.5 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

B)0.5 g PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer and 0.25g lecithin was placed on to a heat block at 100C.

C)0.5 g PVOH (MW:89,000-98,000) with 5 ml of phosphate buffer, 10μL R6G, and 0.25g lecithin was placed on to a heat block at 100C.

D) 0.5 g PVOH (MW:89,000-98,000) with 5 ml of phosphate buffer and 10μL R6G was placed on to a heat block at 100C.

  • Cool down the solutions before homoginizing with safflower oil.
  • Homoginize each of the hydrogels solutions with 50 ml of safflower oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle, 20 hours freeze 4 hours room temperature)

Data

Notes
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Samiye_Yaman/Notebook/Lab_581/2013/02/22&oldid=680144"