User:Samiye Yaman/Notebook/Lab 581/2013/02/20: Difference between revisions
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===Objections== | |||
==Description== | ==Description== | ||
* | *Prepare 3 sets of hydrogels with phosphate buffers | ||
A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C. | |||
B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. | |||
C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. | |||
*Add 2μ of R6G were added for each ml of phophate buffer. Add 6μ R6G in to B and C. Add 8μ to A. | |||
*Homoginize each of the hydrogels solutions with 50 ml of mineral oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle) | |||
==Data== | |||
==Notes== | |||
Revision as of 06:39, 20 February 2013
 Experimental Chemistry
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=ObjectionsDescription*Prepare 3 sets of hydrogels with phosphate buffers A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C. B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
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